This too is consistent with the notion that Citrullus vulgaris Sugar Baley Small these compounds are lipidlike Lycopersicon esculentum Early Hi Crimson ca. RF values of A. The potato leaf died 2 days after toxin injection. Lower Left Insensitive reaction arrow by clone to treatment with 10 Ml of semipurified toxin 14 MgIlil.
After 2 days, typical toxin-induced symptoms developed on the right but not on the left side of the leaf. No reaction resulted when substance 2 was used by itself or when both were used on a resistant clone such as All other compounds shown in Fig. This seems to be the case nation with one another for biological activity. Collectively, Fig. The chemical structures of sub- Among the most prominent of these compounds is alternaric stances 1 and 2 remain to be established, although their solu- acid.
The concept that a phytotoxin or an analogue of a phytotoxin Furthermore, the pathogenicity of A. USA 75 - current technology developed from earlier results is encour- aging 7. From the results of this study, it is clear that variant toxin reaction types insensitive -- highly sensitive were maintained through two somatic generations in regenerated potato plants and were generally consistent in greenhouse and field-grown solvent front plants.
Overall, the evidence presented is suggestive that stable variants of potato differing in toxin sensitivity may be devel- oped through the mesophyll protoplast technique. While the selection for disease resistance is a significant goal, it will be necessary simultaneously to preserve the desirable horticultural traits that characterize the cultivar.
The fact that disease re- sistance was passed through tubers to progeny plants is strongly suggestive that the variation was of genetic origin. Unfortu- nately, the possibility that epigenetic factors might be involved cannot be discounted until segregation of the trait s is examined among sexual progeny.
C The authors acknowledge the help of Dr. Dallas Batchelor, Dr. Roger Totten, Mr. Ray Guthrie, Mr. Ross Beier, and Mr. Robert Johnston in certain technical aspects of this project. A portion of this manuscript was prepared by G. Depart- Cz C ment of Agriculture. Support for U.
Some support for this work was also VK.. I:i 1. Thomas, H. TLC plate illustrating the separation of phosphoric 2. Brian, P. Harvan, D. Rodricks, J. American Chemical Society, Washington, DC , plants regenerated from immature embryos has been reported pp. There have been numerous reports on the use of toxins for 4.
Gilchrist, D. Because of the Hiroe, I. Tottori University, Japan from potato protoplasts 7 , it may be possible to select improved 11, The correlation coeffi- 6. Okuno, I. Shepard, J. Diener, U. Plant Pathologists Pocketbook Commonwealth My- lection of a clone resistant to early blight. However, at least one cological Institute, Kew, Surrey, England. Lukens, R. Table 1. This suggests that factors in addition to the toxins may Silverstein, R. Environmental parameters, microfloral com- York.
Carlson, P. Gengenbach, B. USA 74, The process of screening for disease resistance should be Strobel, G. Plant Physiol. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques.
Membership feels like a huge effort towards a strong, diverse community of people who care about their Education. Your email address will not be published. Save my name, email, and website in this browser for the next time I comment. Skip to content Uncategorized. It is a general purpose medium used to cultivate yeast and mold. To inhibit growth a certain type of acid or antibiotic is used. It is used for different purposes such as:. Image 1: The image on the left shows A.
Picture Source: encrypted-tbn0. Image 2: The photo above is the standard potato dextrose agar plate. It consists of dextrose and dehydrated potato infusion, which encourage luxuriant fungal growth. The solidifying agent is agar.
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